diff --git a/pkg/features/kube_features.go b/pkg/features/kube_features.go
index df6caa21b46..f7e8cbb130b 100644
--- a/pkg/features/kube_features.go
+++ b/pkg/features/kube_features.go
@@ -1232,6 +1232,7 @@ var defaultVersionedKubernetesFeatureGates = map[featuregate.Feature]featuregate
 	},
 	EnvFiles: {
 		{Version: version.MustParse("1.34"), Default: false, PreRelease: featuregate.Alpha},
+		{Version: version.MustParse("1.35"), Default: true, PreRelease: featuregate.Beta},
 	},
 	EventedPLEG: {
 		{Version: version.MustParse("1.26"), Default: false, PreRelease: featuregate.Alpha},
diff --git a/pkg/kubelet/kubelet_pods.go b/pkg/kubelet/kubelet_pods.go
index 67791e17109..e95202d1506 100644
--- a/pkg/kubelet/kubelet_pods.go
+++ b/pkg/kubelet/kubelet_pods.go
@@ -932,12 +932,6 @@ func (kl *Kubelet) makeEnvironmentVariables(pod *v1.Pod, container *v1.Container
 					return result, fmt.Errorf("failed to get host path for volume %q: %w", volume, err)
 				}
 
-				// Validate key length, must not exceed 128 characters.
-				// TODO: @HirazawaUi This limit will be relaxed after the EnvFiles feature gate beta stage.
-				if len(key) > 128 {
-					return result, fmt.Errorf("environment variable key %q exceeds maximum length of 128 characters", key)
-				}
-
 				// Construct the full path to the environment variable file
 				// by combining hostPath with the specified path in FileKeyRef
 				envFilePath, err := securejoin.SecureJoin(hostPath, f.Path)
@@ -951,12 +945,6 @@ func (kl *Kubelet) makeEnvironmentVariables(pod *v1.Pod, container *v1.Container
 					return result, fmt.Errorf("couldn't parse env file")
 				}
 
-				// Validate value size, must not exceed 32KB.
-				// TODO: @HirazawaUi This limit will be relaxed after the EnvFiles feature gate beta stage.
-				if len(runtimeVal) > 32*1024 {
-					return result, fmt.Errorf("environment variable value for key %q exceeds maximum size of 32KB", key)
-				}
-
 				// If the key was not found, and it's not optional, return an error
 				if runtimeVal == "" {
 					if optional {
diff --git a/test/compatibility_lifecycle/reference/versioned_feature_list.yaml b/test/compatibility_lifecycle/reference/versioned_feature_list.yaml
index a724298c088..9fce9afff62 100644
--- a/test/compatibility_lifecycle/reference/versioned_feature_list.yaml
+++ b/test/compatibility_lifecycle/reference/versioned_feature_list.yaml
@@ -563,6 +563,10 @@
     lockToDefault: false
     preRelease: Alpha
     version: "1.34"
+  - default: true
+    lockToDefault: false
+    preRelease: Beta
+    version: "1.35"
 - name: EventedPLEG
   versionedSpecs:
   - default: false