diff --git a/tox.ini b/tox.ini
index 11f83f272..2834ef9f9 100644
--- a/tox.ini
+++ b/tox.ini
@@ -58,7 +58,6 @@ commands =
     {[base]install_and_test} {[base]all_packages}
     python tests/lock_test.py
 setenv =
-    PYTHONPATH = {toxinidir}
     PYTHONHASHSEED = 0
 
 [testenv:py27-oldest]